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On the other hand, activation of the EP2 receptor by its agonist, such as butaprost, a structural analogue of PGE 2 , leads to the formation of the -arrestin1-Src complex, which activates the EGFR and its effectors, including p-ERK1/2, phosphorylated signal transducer and activator of transcription 3 (p-STAT3) and phosphorylated protein kinase B (p-Akt), and also increases the activity of cyclic AMP-dependent protein kinase (PKA) and its downstream effectors, including phosphorylated glycogen synthase kinase 3 (p-GSK3), p-CREB and p-ERK1/2

by polynucleotide reassembly &/or polynucleotide site-saturation mutagenesis) genes from these cells are isolated

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