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Caution: May interact with substances that affect antioxidant balance

MCE Cell Ferrous Iron (Fe 2+ ) Assay Kit (Fluorometric) utilizes fluorescence detection technology to analyze the levels of ferrous ions (Fe 2+ ) in cells

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Cell culture RAW 264.7 murine macrophage cells (TIB-71, American Type Culture Collection) were cultured in DMEM (4.5 g/L d-glucose) supplemented with 10% (vol/vol) FBS, 100 units/mL penicillin and streptomycin (Gibco) at 37 C with 5% CO 2 in humidified air as previously stated in 21

Our results indicate that DDA complexes with GSH-AuNCs and readily induces their complete transfer to chloroform or dichloromethane (typical solvents to prepare PLGA nanoparticles) as well as to other hydrophobic solvents (hexane and toluene) as shown in Fig