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Merck KGaA, Darmstadt, Germany), MS settings of the screening method used in 20202023 were switched to high-mass detection (m/z 7003,000) and the ESI-L LCMS Tuning Solution (Agilent Technologies) was used for calibration, whereas, in the screening method used in 2024 the data acquisition range was changed to m/z 1003,000, and a sodium iodide solution was used for MS calibration

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The earliest and most noticeable indication that B12 is working appears as improved energy levels

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