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Manning MC, Chou DK, Murphy BM, Payne RW, Katayama DS

Several studies have linked B12 deficiency to obesity and a review concluded that B12 deficiency could also lead to a higher percentage of fat accumulation

The following primary antibodies (all from Cell Signaling Technology, Danvers, MA, USA) were used: anti-P-m-TOR (1:1000 dilution, Cat number #5536), anti-m-TOR (1:1000 dilution, Cat number #2983), anti-Beta Actin (1:1000 dilution, Cat number #3700), anti-cleaved PARP (1:2000, Cat number #94885), anti-cleaved caspase 3 (1:1000, Cat number #9661), anti-p62 (1:1000, #88588), anti LC3 (1:1000, Cell signaling technology Cat number #3868) for overnight at 4 C

Invitrogen SuperScript IV VILO Master Mix (Thermo Fisher Scientific) was employed to convert 250 ng of total RNA into cDNA as per the manufacturers instructions, including prior genomic DNA removal with kit-supplied ezDNase

Psychiatry Res 130, 19

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