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If you already have a validated antibody for your protein of interest, then you could perform these experiments with that antibody

doi: 10.3168/jds.2011-5066 23 BradfordMM

After infection, cells were selected in puromycin (1.5 g/ml) and further passaged in culture for functional assays

Phorbol 12-Myristate 13-Acetate Induced Toxicity Study and the Role of Tangeretin in Abrogating HIF-1-NF-B Crosstalk In Vitro and In Vivo

lactic acid builds up in the muscles, causing the muscles to have that burning feeling, but oxygen levels are not affected

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