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release of neurotransmitters, cell-to-cell signalling, cell adhesions, apoptosis, ionic transportation, enzyme regulation as well as protein covalent modifications, such as folding and maturation

However, this may change dramatically under some conditions, e.g., in patients with high-dose methotrexate therapy, an anticancer drug that, by inhibiting dihydrofolate reductase, inhibits the tetrahydrofolate-catalyzed recycling of methionine from homocysteine

J Immunol Methods (2013) 390(1-2):928

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Hence, it had undergone negative selection against self-antigens within the thymus of the donor, and, not surprisingly, we did not observe any off-target activity in the patient