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After 15-20 punctures, the stopper may not reseal properly, compromising the sterile environment

The rate constants from our deglutathionylation assays between 6.4 10 4 and 1.4 10 6 M 1 s 1 are very similar to the rate constants for PfGrx or plant and mammalian glutaredoxins from discontinuous or coupled enzymatic assays with other high and low molecular weight GSSR substrates (Table 1) 9,11,14,19 , thus confirming our assay as an alternative reliable method for the direct analysis of enzyme-catalyzed deglutathionylations

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These compounds were used in the study to block the efflux of gemcitabine mediated by ABC transporters such as P-gp and MRP1, thereby restoring tumor cell sensitivity to drugs and reversing drug resistance

To facilitate its clinical translation, future studies should integrate advanced drug delivery systems, omics-based analyses, and rigorous clinical trials to optimize its pharmacokinetic profile and ensure safety

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