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In addition, this experiment showed that transformation was only possible in fatty acid-containing media (either free fatty acids (FFAs) or lipoproteins 36 ), and that FASN lox/lox -PyMT-HER2 or PyMT-KRAS MEFs could not form colonies in fatty acid-free media (Supplementary Fig

Lipid peroxide measurement Cells were seeded in 6-well plates at 0.5 million cells per well and incubated overnight

Lipid advanced glycosylation: pathway for lipid oxidation in vivo

Bacteria in different groups were collected and diluted 20-fold in MilliQ water at different time intervals

Notably, the increase in incidence of IBD parallels the increase in dietary intake of -6 PUFAs such as AA, which is a major component of a Western diet and contained in meat and eggs 19