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Addition of Trifluoroacetic Acid to Maintain the Acidic Properties of the Analytes Based on the literature, an acidic environment positively impacts analytes (maintaining an acidic environment prevents the oxidation of GSH to GSSG)

[DOI] [PubMed] [Google Scholar] 34.Ahola T., Fellman V., Laaksonen R., Laitila J., Lapatto R., Neuvonen P., Raivio K.O

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As our bodies age, and depending on our environmental conditions, our natural abilities to produce many compounds like glutathione can slow down and become weaker