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Subsequently, a 2% (v/v) inoculum (2 mL) was used to inoculate a fresh 100 mL TSB broth, which was then incubated for an additional 5 h at 37 C to reach the logarithmic phase

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This avenue of translational research also has the potential to further clarify UPF's contribution to ASD risk, which could have significant public health implications, thereby informing dietary recommendations for children, adolescents, and pregnant women during critical neurodevelopmental windows