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3) After IMAC, the eluted peak was immediately desalted (column: HiPrep 26/10, from GE healthcare) in buffer A: 10 mM NaP i at pH 8, 30 mM NaCl, 1 mM GSH, 10% glycerol, and 0.1% reduced Triton X-100

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Under normal conditions, it exists in a benign, non-invasive budding yeast form, held in check by competing beneficial bacteria (predominantly Lactobacillus and Bifidobacterium species), a functional gut immune barrier, and adequate nutritional status