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These monoclonal antibodies bind and inactivate circulating tumour necrosis factor (TNF) alpha, disrupting the inflammatory cascade with a more targeted approach than corticosteroids and thiopurines[71]

Quantification of IL-6, IL-8, IL-1 and PGE2 by enzyme-linked immunoassay (ELISA) Supernatants obtained from the qPCR assay were centrifuged at 600 g for 6 min and used to estimate concentration using bovine IL-8 ELISA kits (#3114-1A-6, Mabtech, Nacka, Sweden), IL-6 (#ESS0029, Thermo Fisher Scientific), IL-1 (#ESS0027, Thermo Fisher Scientific) and PGE2 (#514010, Cayman Chemicals, Ann Arbor, MI, USA) according to the manufacturer's instructions

Common Research Protocols Administration route: Typically delivered as a subcutaneous injection (under the skin), similar to other peptides

The Associated Press Health and Science Department receives support from the Howard Hughes Medical Institutes Department of Science Education and the Robert Wood Johnson Foundation

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