pGEX series expression plasmid Plasmid size: 4969bp Plasmid label: N-GST, N-thrombin Prokaryotic resistance: ampicillin Amp Cloned strain: DH5 alpha Culture conditions: 37 centigrade, aerobic, LB Expression host: Escherichia coli Inducement: IPTG or lactose and its analogues 5'sequencing primers: pGEX5: GGGCTGGCAAGCCACGTTTGGTG 3'sequencing primers: pGEX3: CCGGGAGCTGCATGTGTCAGAGG Note: the recombinant protein can be purified by GST affinity column Use:pGEX Plasmid pGEX-4T-1 Description PGEX-4T-1 is a E.coli expression plasmid pGEX-4T-1 Sequence LOCUS Exported 4969 bp ds-DNA circular SYN 03-8-2015 DEFINITION synthetic circular DNA KEYWORDS pGEX-4T-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4969) TITLE Direct Submission JOURNAL Exported 2015-8-3 FEATURES Location/Qualifiers source 1..4969 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 183..211 /note="tac promoter" /note="strong E
Some studies have suggested that L-Carnitine may help improve sleep by reducing the number of episodes in cyclic vomiting syndrome and by reducing agitation in Alzheimers patients
These findings suggest that Ca 2+ could stimulate mitochondrial DNA transcription and replication by inducing TFAM, potentially promoting mitochondrial biosynthesis
Discovery and biological role of GHK peptide
Franklin CC, Backos DS, Mohar I, White CC, Forman HJ, et al
Annals of Clinical Biochemistry, 43(5), 344- 353
