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The real-time PCR reaction was carried out using a SYBR Green PCR kit (Takara, RR820)

The (His) 6 -tag was cleaved using overnight TEV digestion, followed by ion-exchange chromatography, and finally SEC using a Superdex 200 column (HiLoad 16/600, Cytiva)

Studies indicate that dynamic changes in renal volume (TKV) in AKI patients correlate closely with chronic subclinical damage [283]

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doi: 10.1111/j.1365-2362.2007.01772.x 46