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Changes in absorbance per minute were converted to (moles of conjugated substrate min -1 mg enzyme -1 using the molar extinction coefficient for CDNB 340 = 9.6 mM 1 cm 1

To investigate whether ROS was host or pathogen generated, a separate experiment was conducted in which cells were infected with 1 MOI zoospores (T#5), and either: 1) supplemented with 2.5 mM cysteine on day 3 for 8 hr, 2) incubated at 27C for 8 hr, 3) supplemented with 1.5 mM cysteine and incubated at 30C for 6 hr

Chem Res Toxicol 10(8):895905 Ceni E, Mello T, Galli A (2014) Pathogenesis of alcoholic liver disease: role of oxidative metabolism

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The patient had been quite healthy during a previous visit a month earlier when he received immunizations and prescriptions for antimalarial drugs