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Afterwards, the cells were washed 3 times with PBS, scraped in 500 L of a lysis solution, containing 100 mM ammonium bicarbonate (Sigma Aldrich, Germany) and 1% (w/v) sodium deoxycholate (Sigma Aldrich, Italy), and lysed on ice to prevent protein degradation by intracellular proteases

PHA-665752 is a potent, selective and ATP-competitive c-Met inhibitor, PHA-665752 inhibited c-Met tyrosine phosphorylation at the activation loop (pY1230/34/35), multifunctional docking site (pY1349), and the juxtamembrane domain (pY1003) at 0.1 M [80]

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