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After 8 h, cells were washed with PBS, lysed with RIPA buffer, and sonicated to release all intracellular trehalose

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The opening song The Golden Hour (a reference, in this case, to the importance of swift medical attention within he first hour of an incident) takes on a wider context and personal viewpoint here with the realisation that I have no strength and I have no power / Ive stood sentry at the tower of strong for far too long / So let me in / Let the healing begin. That realisation that the time has come to try to heal the internal pain on its own terms

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