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It is important to review the peripheral smear and hemogram along with HPLC before arriving at a conclusion because though HPLC is the gold standard in diagnosing hemoglobinopathies, the morphological changes in RBCs can only be ascertained on PS

However, consuming particularly high doses of vitamin B12that is, more than the recommended 2.4 micrograms per daycan cause side effects headaches, fatigue, weakness, and tingling in extremities to gastrointestinal symptoms such as diarrhea, nausea, and vomiting