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Quantification of PDC was performed from its chromatographic peak area using extinction coefficient e = 6200 M 1 .cm 1 at 313 nm reported by Michinobu et al

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doi: 10.1002/glia.23607 63 FortinJ

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GPX4 expression was induced by addition of IPTG (1 mM) at an OD 600 of 0.6, and the cells were further cultured at 30C for 5 h