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The lipid mixture was either stored at 80 C or immediately extruded using an Avanti Polar Lipids Mini Extruder (610023) at least 41 times through a 200-nm filter (Whatman Nuclepore Track-Etched Membranes, diameter of 19 mm) for mTORC1 kinase activity assays and cryo-EM studies

J Natl Cancer Inst 106(9), 2014

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Type II collagen (COL II) is one of the major components of the cartilage extracellular matrix (ECM), and the distribution of COL II could reflect neocartilage formation directly in the defect area