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[DOI] [PMC free article] [PubMed] [Google Scholar] 23.Mehendale H.M

The cDNA was subcloned into the pET21a 19 or pET28a (this study) plasmid using the NdeI and SacI restriction sites for the insertion to generate the pET21hHsp70 and pET28hHsp70 plasmids, respectively

Methodology Active Site Identification The active site inside the co-crystallized complex of glutathione reductase (GR) was predicted in using the CASTp (Computed Atlas of Surface Topography of Proteins) online server (Tian et al., 2018)