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Among various bioanalytical techniques Such as quantitative RT-PCR, hybridization assays, HPLC, And LC-MS stem-loop quantitative RT-PCR has become the favored method due to its Superior sensitivity, specificity, extensive dynamic range, And capacity to detect small RNAs as short as 19 bases, rendering it optimal for evaluating the biodistribution and kinetics of RNAi therapeutics

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doi: 10.5772/INTECHOPEN.68695 182 UnverT.ErenlerA