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In a separate study involving 20 healthy individuals, MPs were detected in 90% of the participants via FTIR spectroscopy, exhibiting up to 24 different polymer types, predominantly PE, ethylene propylene diene (EPDM), and ethylene-vinyl-acetate/alcohol, at concentrations between 1.84 and 4.65 g/mL [92]

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To establish suitable targets for the locus-specific assays, we performed genome-scale DNA methylation analysis with the Infinium 450k assay and selected 48 differentially methylated CpGs that cover a broad range of technical challenges encountered in biomarker development (Supplementary Table 3)