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.2020.11.011 PubMed PMID: 33242662

Samples were incubated for 1 h and centrifuged at 14,000 g at 4 C for 20 min

Presenting as macules/papules on the palms and palmar aspects of fingers, these lesions are often painful and cause significant morbidity (Figure 5B)

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To assess whether Cas12f delivery via EVs requires MIM, we attenuated protrusion formation by introducing a five-lysine-to-alanine (5KA) mutation in the MIM I-BAR domain 54 , which was previously shown to impair EV generation 40