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Megaloblastic anemia of the pernicious variety is brought on by a deficiency in the intrinsic factor, which prevents the absorption of vitamin B12

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Due to the instability of vitamin B12, the addition of other medicinal products may result in the vitamin becoming less effective

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For IMR-5/75, the culture media was supplemented with 5 g/mL Blasticidin (Bio-Sciences) and 50 g/mL Zeocin (Thermo Scientific) for plasmid selection