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The durability reflects structural changes (new mitochondria, upregulated AMPK signaling) rather than acute pharmacological effects

PetrenkoACarnevaleMSomovAOsorioJRodrguezJGuibertEet alOrgan preservation into the 2020s: the era of dynamic intervention

Many procedures to perform the biochemical assays developed for other species (Ellman et al., 1961

In vitro, it induces gene mutations in bacteria and fungi and sister chromatid exchanges in mammalian cells (section 7.6)

Cells were collected by centrifugation (500 g, 5 min), washed with PBS, and fixed in 4% paraformaldehyde for 10 minutes at room temperature

Naeem A, Prakash R, Kumari N, Ali Khan M, Quaiyoom Khan A, Uddin S, Verma S, Ab Robertson A et al (2024) MCC950 reduces autophagy and improves cognitive function by inhibiting NLRP3-dependent neuroinflammation in a rat model of Alzheimers disease