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The role of lifestyle interventions such as regular exercise, dietary modifications and weight management in maintaining MHO status remains critical

In practice, combine species reporters that resolve flux and locatione.g., DHE-HPLC to quantify O 2 specifically via 2-OH-E (not raw fluorescence), compartment-targeted HyPer variants to track H 2 O 2 in the matrix/IMS/ER/nucleus and NO/ONOO probes applied with kinetic discrimination and appropriate scavenger/enzyme controlswith footprints of damage that integrate exposure over time (3-nitrotyrosine for nitrative stress, lipid peroxides/F 2 -isoprostanes or MDA/HNE adducts for membrane injury and protein carbonyls for irreversible protein oxidation) (Murphy et al

Kelley, N., Jeltema, D., Duan, Y

BSO increased the mRNA expression of genes associated with GSH biosynthesis ( Gclc, Gclm, Gss , and Cth , Fig