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Cell viability assay Cell proliferation was detected via a CCK-8 assay (Biosharp, China) or a CellTiter-Glo assay (Promega) according to the manufacturers instructions

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Step 4: Insert the needle at a 45 to 90 degree angle, depending on the thickness of the skin fold

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If the protocol lacks precision in dosing, timing, and endpoint measurement, even a mechanistically sound stack will produce inconclusive data

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Apocynum venetum leaf extract alleviated doxorubicin-induced cardiotoxicity through the AKT/Bcl-2 signaling pathway

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Varshney P, Yadav V, Saini N

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