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A 20% loss of initial body weight was selected as a humane endpoint for mice in the SURV experiment as previously described [17]

This process is identical to standard peptide reconstitution but the concentration math is specific to AOD 9604 vial sizes

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After a favorable initial response, the proper maintenance dosage should be determined by decreasing the initial dosage in small amounts to the lowest dosage that maintains an adequate clinical response

48, 49 However, claudin-2 also serves other diverse functions including paracellular passage of cations (including sodium and calcium), cell proliferation and autophagy, and loss of claudin-2 along with claudin-15, yet another TJ protein implicated in paracellular Na + passage, results in premature death because of malnourishment in mice