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The fat and villi were removed, and the samples were incubated at 4 C for 15 min to isolate the crypts

8 , 9 , 41 , 42 , 55 , 65 , 66 Cross-reactive antibodies between gluten/wheat and human tissue epitopes might induce ADs or organ pathology by molecular mimicry

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The EmGFP BLOCK-iT PolIII miR RNAi Expression Vector kit (Thermo Fisher Scientific) was used to design and generate control and rat DP-specific oligonucleotides, which were cloned into the pAd CMV/V5-DEST vector using Gateway recombination (target sequences: 5-AAACCGGAAACATCATCTCTT-3 and 5-TGGTAATAGTTGACCCAGAAA-3)