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It depends on the peptide and the outcome being measured

The stapled peptide they used involved olefinic amino acid Fmoc-( S )-2-(4entenyl)-alanine bound at suitable i , i + 4 positions at the non-binding interface as the staple, and it was found that both selected stapling peptides LCIP1 and LRIP4 exhibited improved cell permeability as compared to the native one in a dose-dependent manner monitored by flow cytometry [130]

129 MorlandC.LauritzenK

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