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To quantify the apoptosis of transplanted human MSCs, the area of colocalized immunolabeling signals (in yellow-orange pixels) of active caspase-3 (in green color) and human nuclei (in red color) in the merged files was measured using ImageJ program and presented as the percentage of colocalization = the area of yellow-orange pixels/total area of red pixels [42]

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Encourage a positive attitude about achieving therapeutic goals