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For CRISPR/Cas9 mediated knock out of both C/EBP isoforms, guide RNA squences (LAP: 5-GAGTGGCCAACTTCTACTACG-3, LIP: 5-GCGCTTACCTCGGCTACCAGG-3) were cloned into pSpCas9(BB)-2A-puro (PX459) v2.0 ( Transfection Immortalized MEFs were transfected with an empty, rat C/EBP-LIP or -LAP containing pcDNA3 or pSV2Zeo vector by using FugeneHD (Promega) according to the manufactures protocol

Sometimes we may not all be able to take an hour-long lunch, but for that brief time that you do have, be satisfied with the food on your plate

In addition, radioactive iodine uptake tests, one of the common ways of assessing thyroid activity, are not always diagnostic, especially in case presentations that are atypical