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Description

R., Maheshwari, A., Nusrat, A

At the end of the incubation the assay plate was equilibrated at room temperature for approximately 30 minutes prior to the addition of 25 uL of CellTiter-Glo 2.0 Reagent

Pittenger, M

Coli species are common culprits in SIBO (small intestinal bacterial overgrowth) issues which I will discuss further in the next section

Membranes were pre-incubated in a 3% bovine serum albumin/Tris buffered saline with Tween 20 solution at room temperature for 30 min to block non-specific binding, followed by overnight incubation at 4 C with rabbit anti-rat interleukin (IL)-6 primary antibody (1:2000 dilution