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Ethics statement The studies involving human participants were reviewed and approved by the Research Ethics Committee of the Second Affiliated Hospital of Chongqing Medical University, China (202164)

BSO treatment experiments An UltiMate 3000 RSLCnano liquid chromatography system (Thermo Fisher Scientific), with a 50 cm PAC Neo HPLC analytical column (COL-NANO050NEOB) and a 0.075 20 mm trap cartridge (Acclaim PepMap C18 100 , 3 m), was connected to a SilicaTip emitter

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Methods used for studying human microbiome, in Probiotics, prebiotics, synbiotics, and postbiotics: human microbiome and human health