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Caspase-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)

Hi Mr_E

Breathlessness: Feeling winded after minor exertion due to a lack of healthy red blood cells to carry oxygen

For homocysteine and methylmalonic acid concentrations, there is a large variability in the cutoff values and combinations with other markers [11]

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