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SIRT3 overexpression in HSCs improves the function of distant tissues through myeloid cells Adoptive transfer experiments demonstrated that splenocytes from aged mice were sufficient to induce systemic senescence in young recipients, despite the short-lived nature of donor immune cells, supporting the concept that relatively small numbers of functionally altered immune cells can exert broad physiological effects through systemic signaling mechanisms, without requiring long-term engraftment 52

Third, while bulk proteomics provides a comprehensive view of cellular differentiation pattern, more precise methods such as single-cell technologies (transcriptomics or proteomics) 38,39 and spatiotemporal single-cell tracking 40,41 would further elucidate the detailed cellular composition and 3D organization during iHLO development

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Its a highly individualized process

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Conservative by design, built on extrapolation from pharmacokinetic modeling rather than pregnancy-specific clearance data