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The proteins in the pulldown were resuspended in sample buffer, samples were resolved by NuPAGE gel electrophoresis and anti-MycMunc13-4 (71D10, Cell signaling) or anti-FlagMunc13-4 (Genecopoeia, CGAB-DDK-0050) and GST (Sigma, 06-332) were detected by western blot

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Impaired protein digestion (such as low stomach acid or pancreatic enzymes) may also be a limiting factor in ensuring healthy Glutathione levels

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Extensive research has indicated that GPX3 is significantly downregulated in OC, and patients with elevated expression levels of this gene in their tumors are more likely to experience a poor prognosis