The libraries were further quantified on a Qubit 2.0 fluorometer using Qubit dsDNA HS kit (Life Technologies, USA) and by quantitative PCR (qPCR) using KAPA SYBR FAST qPCR Master mix and Illumina standards and primer premix (KAPA Biosystems, Wilmington, MA, USA), following the Illumina-suggested protocol
ELISA kits were used to determine the expressions of IL-6, TNF-, and VEGF (CUSABIO, Hubei, China) and IL-1 and HIF-1 (MyBioSource, San Diego, USA)
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M., Fleming, B
