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The very first of these, celluloid, was occasionally used in the production of cigarette and cigar holders, but was discontinued as soon as other plastics became available due to its flammability

Extracellular flux assayglycolysis rate assay Cells were plated at a density of 7500 cells/well in XF96 micro-plates (Agilent Technologies) in DMEM/F12 supplemented with 10% FBS 48 h prior to the assay and cultivated in a humified incubator at 37 C with 5% CO 2

The move was a strategic pivot after the industry was barred from advertising to children and had to abandon ads like the infamous Flintstones commercial where Fred and Barney took a smoking break

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