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Cell viability was determined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay

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So Zyn may offer adult smokers who have not been able to quit smoking a way to reduce their exposure to the toxic chemicals that cause disease, including cancer. But the nicotine in Zyn pouches is highly addictive and it may increase cardiovascular disease risk in people not already using nicotine products, Rees said