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As currently constructed, the model replicates the overall lung volumes, ventilatory characteristics, and environmental physiology of a breathing lung, but lacks the airway and alveolar structures required to emulate the distributive airflow features and particle deposition that are inherent to human lungs 48,49

Nicotine, the principal component of TS and e-cig, can negatively affect tight junctional protein expression, compromising BBB integrity (Abbruscato et al., 2002

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Thus, we extended the pre-registration following the same methods as pre-registered analyses for research question 3 above

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