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A 5 ml mixture containing 1666.7ul of each LDH assay substrate solution, LDH assay cofactor, and LDH assay dye solution was prepared, of which 50 L (standard kit volume) was pipetted into each well containing samples (Figure 2)

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According to WHO, every six seconds, one person dies from a tobacco-caused disease totaling nearly six million people each year - a figure which is expected to rise to more than eight million by 2030