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Acute and chronic NTHI exposure A frozen glycerol stock of NTHI strain 1479 (clinical isolate from a COPD exacerbation) was streaked on chocolate-agar plates and single colonies were grown in a liquid culture of brain-heart infusion media supplemented with 10 g/ml hemin and 10 g/ml -nicotinamide adenine dinucleotide (Sigma)

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