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This study revealed that higher ASC in ripening orange was associated with an augmented expression of four genes involved in ASC biosynthesis, encoding GDP-d-mannose-3,5-epimerase (EC 5.1.3.18), GDP-l-galactose-phosphorylase (EC 2.7.7.69), l-galactose dehydrogenase (EC 1.1.1.316) and l-galactono-1,4-lactone dehydrogenase (EC 1.3.2.3), together with attenuated activities of ASC oxidase and ASC peroxidase, which are involved in ASC degradation

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Purified products as well as reaction mixtures were characterized by analytical LC-MS (Waters 2695 LC/ZQ 2000 quadripole) on a reverse-phase column XBridge BEH300 C18 (3.5 m, 300 , 4.6 150 mm) using a linear gradient of increasing concentration of eluent B in eluent A