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For PCR analysis, 2 L of the lysate was used as a template with the following primers: F1: 5-GCCAGCTACTGCATATATTGAGACC-3 R1: 5-GGATATAGAGAAGCCCTAGAACAGGG-3 R2: 5-CATGTCGTGTTGGTCAAATAAGCAC-3 PCR amplification using primers F1/R1 generated a 278 bp product for wild-type (WT) alleles and a 382 bp product for floxed alleles

Ferritin is unreliable in cases of inflammation therefore transferrin saturation is more reliable but has a 2 hour window for testing after blood draw for accurate results

Its structure is its function

10.1161/CIRCRESAHA.118.313865 62 GreitenL