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45 Degrees vs 90 Degrees: Choosing the Right Angle The 45-degree angle is appropriate when the subcutaneous fat layer is thin, specifically when the pinch technique is used or when injecting at a site with limited adipose tissue

Ita JR, Castilla-Cortzar I, Aguirre GA et al (2015) Altered liver expression of genes involved in lipid and glucose metabolism in mice with partial IGF-1 deficiency: an experimental approach to metabolic syndrome