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Results indicate that ALA treated mice displayed mRNA and protein changes consistent with enhanced antioxidant mechanisms

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Assays were performed following the manufacturers instructions for PRMT5 (AMS biotechnology 52002L) and AHCY (ABCAM ab204694)

The pooled protein was concentrated using Amicon Ultra-15, 30 kDa centrifugal filters and FAD (500 M) was added prior to being stored at 80C