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Zachara, N

Superoxide accumulation was measured using the Total ROS/Superoxide Detection Kit from Enzo Life Sciences (ENZ-51010)

[Products in use] Extracellular LDH : Cytotoxicity LDH Assay Kit-WST (Product code: CK12) Phosphatidylserine: Annexin V Apoptosis Plate Assay KitProduct code: AD12 Cell viability : Cell Counting Kit-8 (Product code: CK04) Intracellular Fe 2+ : FerroOrange (Product cose: F374) *Normalized with Hoechst 33342 fluorescence intensity Lipid peroxidation : Lipid Peroxidation Probe -BDP 581/591 C11- (Product code: L267) [Experimental conditions] Cell type: HepG2 cell(210 4 cells/well) Drugs: Staurosporin(5 mol/l), Erastin(25 mol/l), Erastin+RSL3(both 25 mol/l) *Diluted in serum-free medium

Researchers must balance plasma stability with intracellular release rates, control non-specific early payload release, and address aggregation issues caused by linker hydrophobicity

Nuclei often enlarged

Nat Chem Biol 13:9198 Markovic M, Ben-Shabat S, Keinan S, Aponick A, Zimmermann EM, Dahan A (2019) Lipidic prodrug approach for improved oral drug delivery and therapy