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doi: 10.1021/jacs.9b11454 49 HuangQRuYLuoYLuoXLiuDMaYet al

Metabolic effects of a growth hormone-releasing factor in obese subjects with reduced growth hormone secretion: a randomized controlled trial

Activation of this receptor increases intracellular calcium mobilization, which promotes episodic exocytosis of pre-synthesized growth hormone rather than continuous secretion

The combination allows investigation of dual receptor-pathway activation within GHaxisrelated systems

The precise sequence and length requirements for the PAM differ depending on the Casl3 protein used, but PAMs are typically 2-5 base pair sequences adjacent the protospacer (that is, the target sequence)